毒理学研究与细胞培养系统
来源： 浏览量：673 更新日期：2010年1月20日
Toxicology investigations with cell culture systems: 20 years after.
Zucco F, De Angelis I, Testai E, Stammati A.
Istituto di Neurobiologia e Medicina Molecolare, C.N.R., Viale Marx 15, 00137, Rome, Italy
From almost 20 years the "in vitro" model has gained a wide ground in toxicological investigation, providing advanced tools, reliable protocols, mechanistic information. These advancements have been done thanks to different approaches, addressed at improving chemical testing and validating procedures, at exploring the cellular and molecular basis of toxicity, at studying the modifications that xenobiotics undergo in the cellular environment. In this review the most advanced cellular models, the mechanisms of cell death, the techniques to monitor gene activation, following chemical exposure, is highlighted. Moreover the more recent in vitro models to approach the biotransformation issue will be presented.